columns
of ten buildings with blasting
gelatin. In two minutes, primary
charges will blow base charges, and
those buildings will be reduced to
smoldering rubble. I know this
because Tyler knows this.
TYLER
Look what weve accomplised.
(checks watch)
Thirty seconds.
JACK (V.O.)
Somehow, I realize all of this -- the
gun, the bombs, the revolution -- is
really about Marla Singer.
PULL BACK from Jack
1. 실험 목적
- 각자 채취한 혈액으로부터 DNA 추출 Kit를 사용하여 Genomic DNA를 추출한다.
- Spectrometer(Nano Drop)를 이용하여 추출된 DNA의 Optical Density를 측정하고 이로부터 DNA의 농도를 계산한다.
- DNA의 종류와 DNA 추출 방법을 알아본다.
- 흡광도 측정에 의한 DNA 농도 계산 및 순도측정의 원리를 알아본다.
Nucleic Acid Hybridization Techniques
-------------------------------------
1. INTRODUCTION
* Southern blotting (DNA)와 northern blotting(RNA)의 원리
① nucleic acid 추출 → 제한효소 처리
② negative charge인 nucleic acid의 단편을 agarose gel 의 전기영동에 의해 크기로 분획
③ 분획된 RNA 또는 denatured DNA를 nitrocellulose 또는 nylon membrane에 고정
A silver throwing-bird is chosen from a weapons array. Gloved hands lift a black eye mask. Tunic armor CLICKS shut, turning to reveal the chest-borne insignia of a Robin.
INT. BATCAVE
BATMAN - CLOSE
Emerges from his costume vault.
WIDER
Deeper excavation has doubled the size of the cavern.
New state-of-the-art computing systems flash. Surveillance screens monitor news and police FREQUENCIES. Crim
Trucks are parked on the parking lot of an ice rink. Flemish technicians, grips and electricians, talk together while unloading the equipment, lamps, cables, tracks. They spread it out on the parking lot, take some lamps into the rink. The four technicians all wear the same black T-shirt with an inscription in Dutch.
A few cars arrive, belonging to the film crew (cinematographer, sound engineer).
A SILHOUETTED MAN in front of mirror dons his Marine dress blues. Spit polish shoes laced. Medals clipped to jacket. All in CLOSEUP. We never see him fully.
The Man puts HIS HAT squarely on his head. EYES glint.
On the mans dresser: MARINE MEMORABILIA. Three Purple Hearts, photos of a WOMAN (his wife), PHOTOS OF MARINES in combat locales.
TIGHTER INTO THE PHOTOS
We HEAR PANICKED VOICES, EXPLOSION
DAWN OF THE DEAD
by George A. Romero
모
"WHEN THERE IS NO MORE ROOM IN HELL
모
THE DEAD WILL WALK THE EARTH"
모
모
1 We see the face of a young woman. She is asleep. It is very
quiet at first, as credits appear. The womans face begins to
twitch, as though she is having a bad dream. She moans slightly
and her expression grows more desperate.
A mix of subtle sounds begin to fade in. As they get
The Rock
Screenplay by David Weisberg
Douglas S. Cook
Mark Rosner
Aaron Sorkin
Produced by Don Simpson
Jerry Bruckheimer
Directed by Michael Bay
Cast List:
Nicholas Cage Stanley Goodspeed
Sean Connery John Patrick Mason
Ed Harris Brig. Gen. Francis X. Hummel
David Morse Maj. Tom Baxter
John C. Mc Ginley Marine Capt. Hendrix
Bokeem Woodbine Sgt. Crisp
William Forsythe Eddie Paxton
BEGIN MAIN TITL
1. 개발관련 배경
1960년대, mouse bone marrow과 spleen cells에서 granulocytes 와 monocytes의 두 그룹에서 colony 성장을 자극하는 방법을 발견하였다.
Colony 의 성장은 알고있지 못하는 colony-stimulating factors (CSF)라는 인자에 의존하였다. 생물학적으로 CSF에 대하여 분석하고 생화학적으로 정제하여 1980 년대에 CSF 가